MiR-340 regulates the growth and metabolism of renal cell carcinoma cells by targeting frizzled class receptor 3.
By Mingfeng Xiang,Yanqun Huang,Changjun Dai,Gaode Zou Feb 19, 2021
MicroRNA(miR)-340 is known as a multifunctional miRNA related to various types of cancer while its role in renal cell carcinoma (RCC) remains to be further investigated. In the present study, an apparent increase in miR-340 expression was observed in both clear cell RCC tissues and RCC cell line 786-O and Caki-1. Functionally, the overexpression of miR-340 promoted cell proliferation, migration, invasion, extracellular alanine (Ala) level, and glycolysis level in 786-O cells. Then, frizzled class receptor 3 (FZD3) was determined as the target gene of miR-340 and its expression level was negatively regulated by miR-340. The FZD3 silencing abrogated the inhibitory effect of miR-340 knockdown on cell proliferation, migration, invasion, Ala level, and glycolysis level in 786-O cells. In conclusion, miR-340 promotes proliferation, migration, and invasion of RCC cells via suppressing FZD3 expression, and the promotion effect of miR-340 on RCC progression may be due to its regulatory effect on glycolysis and Ala level.
About The Author
Mingfeng Xiang,Yanqun Huang,Changjun Dai,Gaode Zou
Department of Urology, The Second Affiliated Hospital of Nanchang University, 1 Mingde RD, Nanchang, 310003, China.
School of Inter-Cultural Studies, Jiangxi Normal University, Nanchang, 330022, China.
People’s Hospital of Linchuan District, Fuzhou City, 344000, Jiangxi Province, China.
Department of Urology, The Second Affiliated Hospital of Nanchang University, 1 Mingde RD, Nanchang, 310003, China. Gaode_zou@163.com.