Dyssodia decipiens is one of the seven recognized species within the Dyssodia genus which has economic and social relevance in Mexico. Herein we report on the volatile characterization and pharmacological activity of D. decipiens . The annual essential oils (2017-2019) contained verbenone (30.8-60.9%), limonene (5.6-35.5%), β-caryophyllene (7.8-19.8%) and linalool (4.5-12.4%) as major constituents along the studied years. Antifungal properties were probed on Candida albicans ATCC 90028, ATCC 10231 (fluconazole resistant) and IS1-IS5 (five clinical isolates). Flower essential oils had the best anti- C. albicans activity (MIC, 59.2-93.5 µg mL -1 ), limonene (MIC, 125.5-188.4 µg mL -1 ) and β-caryophyllene (MIC, 104.3-184.2 µg mL -1 ) were involved in this effect. SEM examination revealed that D. decipiens essential oils prouduced an evident lysis on the fungus. Mosquito repellent activity was demonstrated on Aedes aegypti , a vector of dengue, chikungunya and zika viruses. The repellent activity of leaf essential oils (20%) was effective within 1-5 h post-treatment (> 90%) and it was stronger ( p < 0.01) than that of commercial DEET. The evaluation of the four major volatiles (10% each) produced similar results to those of essential oils. Finally, leaf essentila oils showed a moderate antiprolifertive activity on the line OVCAR-3 (LD 50 , 56.5-85.7 µg mL -1 ), verbenone (LD 50 ,65.3 µg mL -1 ) and β-caryophyllene (LD 50 , 43.6 µg mL -1 ) were linked to this effect.© 2020 Wiley-VCH GmbH.