Our goal was compared the efficiency of the CapitalBioMycobacterium real-time polymerase chain reaction (PCR) detection test with standard Xpert MTB/RIF for the diagnosis of tuberculous meningitis (TBM).
We analyzed cerebrospinal fluid (CSF) from 163 patients with suspected TBM that were collected between January 1, 2018, and December 31, 2019. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and area under the curve (AUC) of both tests were determined and their diagnostic accuracies were compared using clinical diagnosis as reference standard.
The sensitivity, specificity, PPV, NPV and AUC of the CapitalBio Mycobacterium detection test were 48.5%, 100%, 100%, 29.6%, and 0.74, respectively, when used for the diagnosis of TBM; values for the Xpert MTB/RIF included 47.0%, 100%, 100%, 29.0%, and 0.74, respectively. The diagnostic accuracies of the CapitalBio Mycobacterium detection test and the Xpert MTB/RIF were similar to one another; both were significantly higher than that associated with acid - fast staining to detect mycobacteria.
The CapitalBio Mycobacterium real-time PCR detection test has moderate sensitivity and very high specificity for TBM, with results similar to those of Xpert MTB/RIF. This test may be used as another initial screening for TBM.
Copyright © 2020. Published by Elsevier Ltd.
Comparison of the accuracy of CapitalBio Mycobacterium real-time polymerase chain reaction and Xpert MTB/RIF for the diagnosis of tuberculous meningitis.
