The present work was designed to assess the potential modulatory and antioxidant conclusions of varied concentration of mulberry Morus alba leaves (MAL) extract supplementation on Nile tilapia (Oreochromis .niloticus). A total two hundred and forty of O. niloticus were haphazardly sorted into four groups. The control (CT) group was fed on basal diet. A group MAL, MAL and MAL was fed on 1, 3 and 5 g/kg MAL respectively for thirty days. On day thirty one, half of replicates in each group were challenged by 0.5 ml ×10Aeromonas hydrophila where, the residual replicates were kept without challenge. A. hydrophila challenged tilapias revealed anemia that alleviated by supplementation with 5 g/kg MAL also, recovers the shift of leucogram prompted by challenge. Elevation of alkaline phosphatase, aminotransferases, lactate dehydrogenase and malondialdehyde (ALP, ALT, AST, LDH and MDA) in CT, MAL and MAL in challenged replicates respectively where within normal at MAL. Supplementation with MAL showed more potent antioxidant and immune reaction than MAL and MAL. There were a rapid increase of immunoglobulin M, lysozymes, nitric oxide, catalase and superoxide dismutase and their allied genes expression (IgM, CAT and SOD) in MAL groups with contrast in CT challenged groups. Where in challenged groups, there was suppression in genes expression of interleukins (8 and 1 beta) and interferon ɤ (IL8. IL-1β and INFɤ). Tilapias challenged by A. hydrophila unveiled plentiful surge in the percentage of mortality in CT challenged fish (80%), followed by the groups supplemented with MAL and MAL were (73.33%) where MAL was 20 %. The mortalities have been halted from the 6, 13, 14 and 15 days in, MAL, MAL, MAL, and CT correspondingly. These previous results could be fulfilled that using of MAL 5 g/kg enhance the immune feedback, antioxidant defense and resistance of tilapia against A. hydrophila.
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