To compare the effect of dynamic (DRT) and isometric (IRT) resistance training on glycemic homeostasis, lipid profile, and nitric oxide (NO) in hemodialysis (HD) patients. Patients were randomly distributed into three groups: control (CTL; n=65), DRT (n=65), and IRT (n=67). Patients were tested for fasting blood glucose, glycated hemoglobin, oral glucose tolerance test, insulin resistance, lipid profile, leptin, insulin, adiponectin, C-reactive protein, and NO were assessed pre- and post-intervention period. Patients underwent to strength and body composition assessments. Subjects allocated in both DRT and IRT groups took part in a 24-week resistance training program, three times per week. Each training session was approximately one hour before dialysis and consisted of 3 sets of 8-12 repetitions at low intensity. Total workload was higher in the DRT as compared with the IRT. This heightened workload related to better glycemic homeostasis in HD patients as measured by regulation of insulin, adiponectin, and leptin, while improvement of triglycerides, free-fat mass, and muscle strength. Additionally, NO levels were increased on DRT group. NO was significantly correlated with glucose intolerance (r=-0.42, p=0.0155) and workload (r=0.46, p=0.0022). The IRT group only improved strength (p<0.05). 24-week of DRT improved glycemic homeostasis, lipid profile, and NO in HD patients. Although IRT seems to play an important role in increasing strength, DRT might be a better choice to promote metabolic adjustments in HD patients. Clinical Trial http://www.ensaiosclinicos.gov.br/rg/RBR-3gpg5w/; nº RBR-3gpg5w Novelty Bullets: -DRT might be a better choice for metabolic improvements in CKD patients. -Exercise-training might treat metabolic imbalance in CKD patients.