The aim of this preliminary study was to determine the prevalence of Bovine Viral Diarrhea Virus (BVDV) and its association with reproductive problems in cattle in Timika, Southern Papua, Indonesia, an emerging area for beef production. Serum from 77 beef cattle was collected from four villages and tested, using both antibody and antigen ELISA kits for BVDV. Data of the villages of origin, age, breed, sex and the number of parities of the cattle were collected. The pregnancy status of the cattle was determined by rectal examination. Results showed that the prevalence of BVDV antibody in individual cattle in Timika was 11.7% (CI: 6.1 – 20.0%), while at the farm level the antibody prevalence was 18.4% (CI: 8.9 – 33.7%).). Seropositivity for BVDV increased with age (P=0.02), from 0% (CI: 0 – 37.2%) in less than two-years old to 28.6% (CI: 11.3 – 55.0%) in cattle older than eight years of age. BVDV antibody was 2.9 times more prevalent in non-pregnant cows than in antibody negative cows (CI: 1.02 – 8.14, P=0.04). BVD antigen was not detected in the present study. This study reported for the first time, evidence of infection with BVDV in cattle in Papua and indicated that BVDV infection may be associated with infertility.Copyright © 2020 Elsevier B.V. All rights reserved.