In the study350 individuals were enrolled, 150 allergic asthma patients and 200 healthy controls.SNP analyses were performed by RFLP. IL-4, IL-13 vitamin D and Total IgE were measured by ELISA.
The AA homozygous mutant of -3279 C/A posed a three-fold risk [P<0.005; OR, 3.52]whereas the -2383C/T variants TT genotype carried a fourfold risk [P =0.002; OR, 4.04].Haplotype analysis exhibited predisposition to allergic asthmawith CC/TT [P=0.01; OR 5.93 (95%CI)], AA/CC [P=0.01; OR 3.29] and AA/TT haplotypes [P=0; OR 11.86 (1.31-85.87)]. A negative correlation between IgE and Vitamin D was found [r= -0.30 p-value 0.001] but a negative correlation betweenIgE and Vit D was established in the haplotype CC/TT [r= -0.45 P=0.002] and CC/CT [r=-0.52 P=0.04].In allergic patients, the eosinophils count was high [p=0.003]and the mean levels of pro-inflammatory cytokines IL-4 and IL-13 were elevated[P<0.001]as well.
The study suggests SNP -3279 -AA genotype and, -2383-TT genotype in association with certain haplotypes pose a risk for allergy development. There was no correlation between different genotypes and serum levels of various cytokines.
Copyright © 2020 Elsevier B.V. All rights reserved.