PD-1 immune checkpoint blockade and cytokine IL-33 have shown significant therapeutic effects in tumor immunotherapy. These therapies promote CD8 T cell activation, proliferation, and effector functions. However, there were few research about the combined therapy efficacy. In this study, we established B16-empty vector and B16-IL33 melanoma mouse models and treated with PD-1 monoclonal antibody. We reported that PD-1 blockade combined with cytokine IL-33 further inhibited tumor progression and prolonged the survival of tumor-bearing mice. Mechanistically, the combination therapy was found to further facilitate CD4 and CD8 T lymphocytes accumulation, and enhance the antitumor effects of CD4or CD8tumor-infiltrating lymphocytes by promoting type-1 immune response within the tumor microenvironment using flow cytometry and quantitative real time polymerase chain reaction. Thus, PD-1 blockade combined with IL-33 has application potential in tumor immunotherapy. Further, this study provides a new promising strategy and theoretical basis for tumor combination immunotherapy.Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.
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Honghong He,Liyan Shi,Dan Meng,Huijun Zhou,Jingshu Ma,Yixian Wu,Yanshi Wu,Yanzheng Gu,Wei Xie,Jing Zhang,Yibei Zhu