The SePSP was extracted using an ethanol precipitation procedure, followed by a DEAE anion-exchange and size-exclusion chromatography. The mass ratio of the polysaccharide and peptide components in the purified SePSP was approximately 4.87:1. By determining its free radical scavenging abilities using DPPH, the hydroxyl free radical, and the superoxide anion free radical, as well as its total reducing power, SePSP had strong concentration-dependent antioxidant activity in vitro. Further, SePSP effectively alleviated dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) in mice. Administration of 200 mg/kg SePSP by gavage for 7 days prevented body weight loss; significantly reduced the mRNA levels of proinflammatory cytokines including, TNF-α and IL-1β; increased mRNA level of the anti-inflammatory cytokine IL-10 in the colon; and decreased the malondialdehyde concentration from 6.42 to 4.82 μmol/L in the blood in UC mice.
The SePSP had strong concentration-dependent antioxidant activity in vitro and effectively alleviated DSS-induced UC in mice. The in vivo therapeutic efficacy in DSS-induced UC may be mediated through modulating the expression of inflammatory cytokines and inhibiting oxidative stress. The findings provide a scientific rational to utilize bioactive nutraceuticals from Sanghuang mushrooms to develop functional foods for the prevention and treatment of UC. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.