Rice ( L.) yield is severely reduced by the brown planthopper (BPH), Stål, in Asian countries. Increasing resistance in rice against BPH can mitigate yield loss. Previous reports indicated the presence of three BPH resistance genes, , , and , in durable resistant rice cultivar ‘PTB33’. However, several important questions remain unclear; the genetic locations of BPH resistance genes on rice chromosomes and how these genes confer resistance, especially with relationship to three major categories of resistance mechanisms; antibiosis, antixenosis or tolerance. In this study, locations of , , and were delimited using chromosome segment substitution lines derived from crosses between ‘Taichung 65’ and near-isogenic lines for (-NIL), (-NIL), and (-NIL). was delimited as approximately 247.5 kbp between RM28449 and ID-161-2 on chromosome 12. and were located between RM1305 and RM6156 on chromosome 4 and RM508 and RM19341 on chromosome 6, respectively. The antibiosis, antixenosis, and tolerance were estimated by several tests using -NIL, -NIL, and -NIL. and showed resistance to antibiosis and antixenosis, while and showed tolerance. These results contribute to the development of durable BPH resistance lines using three resistance genes from ‘PTB33’.