Tacrolimus is an immunosuppressive drug used to prevent organ rejections. Many factors could influence blood concentration of tacrolimus.
To detect genotypes of cytochrome () and in kidney transplant patients and establish initial daily tacrolimus dosing formula based on genotypes of and and patients’ clinical parameters.
Sequence specific primer polymerase chain reaction (PCR) and PCR restriction fragment length polymorphism were used to detect genotypes of and . The blood cell, procalcitonin, C-reactive protein, height, weight, age, gender and other clinical parameters were recorded. Multiple linear regression analysis and Pearson correlation analysis were used to conduct date analysis.
102 cases were enrolled in cohort 1, and there were 10 cases of *1/*1 (9.8%), 28 cases of *1/*3 (27.5%), and 64 cases of *3/*3 (62.7%). The distributions of genotype were 36 (35.3%), 52 (51.0%), and 14 (13.7%). The distributions of genotype were 39 (38.2%), 40 (39.2%), and 23 (22.5%). The formula was 7.499 + (0.053 × Weight) – (0.029 × Hemoglobin concentration) – (1.045 × genotype) ( genotype: *1/*1 type inputs 0, *1/*3 type inputs 1, *3/*3 type inputs 2). The predicted doses from the established formula had a significant correlation ( = 0.605) with actual clinical doses ( < 0.05).
Hemoglobin concentration, weight, and genotype should be considered using tacrolimus. The initial daily tacrolimus dosing formula established can make a good prediction.