Prior studies have noted the importance of T cell immunoglobulin and mucin domain containing 4 (TIMD4) in various diseases and its functions on cell malignant behaviors. However, the biological function of TIMD4 in Diffuse Large B-Cell Lymphoma (DLBCL) is unknown.
Relative expression of TIMD4 was analyzed based on the GSE56315 array including 88 cases of human tissues. TIMD4 expression in cells was detected using qRT-PCR and western blot experiments. Cell proliferation was measured by Cell Counting Kit 8 (CCK 8) assay and apoptotic property was assessed through the detection of related proteins by western blot. The underlying molecular mechanism of TIMD4 in DLBCL was predicted and confirmed using KEGG enrichment analysis and western blot.
Results indicate that TIMD4 is overexpressed in DLBCL tissues and the poor prognosis of DLBCL patients is significantly linked with the higher TIMD4 expression. The loss-of-TIMD4 experiment in CYP6D elaborates that knockdown of TIMD4 blocks cell growth and accelerates cell apoptosis, while the gain-of-TIMD4 experiment in Raji suggests that up-regulation of TIMD4 promotes cell proliferation and inhibits cell apoptosis. Activity of Wnt/β-catenin pathway is mediated by the TIMD4 expression in DLBCL cells.
These findings illustrates that TIMD4 is upregulated in patients with DLBCL and the regulatory effects of TIMD4 on cell proliferation and apoptosis are associated with the Wnt/β-catenin pathway, posing a novel target for DLBCL therapy.

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