Artificial insemination plays an important role in genetic improvement in the goat farming system. The aim of this study was to investigate the effect of cervical application of Hyaluronan (HA) on the fertility in goats after cervical artificial insemination (CAI) using frozen-thawed (F-T semen).
After oestrous synchronisation with progesterone sponges and pregnant mare serum gonadotropin (PMSG) injection, both nulli- and multi-parous goats, were randomly allocated to 2 groups, and were inseminated with 0.25 ml of F-T semen (150 x 106 spermatozoa) twice at 52 h and 56 h after sponge removal. Prior to the insemination, goats in Group 1 only were given topical cervical HA application at 48 h after sponge removal. Site of insemination was recorded as os-cervix or intra-cervix or intra-uterus. Pregnancy was tested ultrasonographically 42 days after insemination. The data on pregnancy rates and percentage of animals according to the site of semen deposition were compared by Chi-square analysis.
The overall pregnancy rate was significantly (p<0.004) higher in goats with prior application to the cervix with HA (63.3%) than without (36.0%). Same pattern was observed in the pregnancy rates of nulli- and multi-parous goats in both the groups. Percentage of nulliparous goats according to the site of insemination in the HA group did not differ between first and the second insemination. However, in multiparous goats the percentage of animals inseminated intra-cervically was significantly increased (p≤ 0.05) between the first and the second inseminations.
The results suggest that significantly higher fertility rate in the “HA goats” compared to the “without HA” group was because of deeper insemination facilitated by topical cervical application of HA. The deeper insemination into the cervical canal increase the rate of fertilisation when the cervical artificially insemination is performed.